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      小鼠氚水內污染誘導的DNA甲基化改變
      來源: | 作者: | 發布時間:2013-11-11 17:58:57

      小鼠氚水內污染誘導的DNA甲基化改變
      鄭璐琳1,黃鋮鋮1,孫秀錦1,包廣糧2,孫亮1,陳秋1,崔鳳梅1
      基金項目:基金項目:國家自然科學基金(30800922,81172706) 作者簡介:鄭璐琳(1987年出生), 女, 在讀研究生, 放射毒理學方向 通信聯系人:崔鳳梅(1975年出生), 女, 副教授, 放射毒理學方向.

      (1. 蘇州大學醫學部放射醫學與防護學院,蘇州 215123; 2. 中國科學院上海應用物理研究所,上海 201800)
      摘要:本文建立氚水染毒小鼠模型,采用甲基化DNA免疫沉淀-芯片(MeDIP-chip)雜交技術分析小鼠肝組織基因啟動子CpG甲基化情況,并檢測了肺、肝、腎臟組織DNMT1的表達水平。結果顯示,不同濃度氚染毒10 d的小鼠肝臟組織基因組出現了新的甲基化位點,而且高濃度和低濃度染毒組的共同發生甲基化的基因有19個,17個發生在啟動子的CpG。隨著氚水10 初始注入量增加,累積劑量增大,肺、肝、腎組織DNMTl表達量相應降低。氚水初始注入量為5.55×105 Bq/g時,隨注入后時間延長,累積劑量增大,DNMTl表達量下降后再恢復。實驗結果提示,甲基化改變的差異基因可能在氚水內照射損傷時起調控作用,不同組織中DNMT1的檢測可用于輻射損傷的早期檢測或損傷恢復的評估。

      關鍵詞:DNA甲基化;氚;DNMT1
      中圖分類號:R818.03
      The change of DNA methylation induced by HTO internal exposure in mice
      Zheng Lulin1, Huang Chengcheng1, Sun Xiujing1, Bao Guangliang2, Sun Liang1, Chen Qiu1, Cui Fengmei1
      (1. School of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215123; 2. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 200000)

      Abstract: We established a mouse model of internal radiation of triated water(HTO). The methylation levels of promoter CpG were assessed by immunoprecipitation-chip (MeDIP-chip) hybridization of DNA methylation and the expression of DNA methyltransferase(DNMT1)were detected in different organs. The results showed some genes were getting methylated in mouse liver tissue 10 days after injection with different concentration of HTO, and there were the 30 same 19 genes getting methylated in both high and low concentration groups, 17 of them were at promotor CpG. With the increase concentration of HTO, the expression level of DNMT1 decreased in lung, liver and renal tissues. And when the concentration was 5.55×105 Bq/g, as time moved on, cumulative dose increased, the expression level of DNMT1 decreased and then went up. These results indicated those genes getting methylated probably be involved in regulating the 35 process of radiation damage caused by HTO, and DNMT1 expression level in different organs could be used for early detection of radiation damage or evaluation of damage repairing.

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