• <rp id="hvkkj"><menuitem id="hvkkj"></menuitem></rp>

    1. <bdo id="hvkkj"></bdo>
      <b id="hvkkj"><small id="hvkkj"></small></b>

    2. <tt id="hvkkj"></tt>

      1. 當前位置:首頁 > 智慧健康列表 > 腦科學和醫療技術 > 詳細信息
        流體剪切力對喉鱗癌Hep2 細胞通透性的影響
        來源: | 作者: | 發布時間:2013-11-11 18:03:31

        流體剪切力對喉鱗癌Hep2 細胞通透性的影響
        周法庭,劉肖珩,沈陽,劉雙鳳,洪錦勇,夏慶
        基金項目:*教育部高等學校博士學科點專項科研基金(20120181120058),國家自然科學基金資助項目
        (11372203,11172189),國家基礎科學人才培養基金能力提高資助項目(J1103604)。
        作者簡介:周法庭 男,碩士在讀, 主要研究方向:腫瘤細胞信號系統與作用靶標
        通信聯系人:劉肖珩 男 1968 年出生, 教授, 博士生導師。主要研究方向:⑴ 組織、細胞生物力學與工程及臨床應用; ⑵ 組織工程與人工器官;⑶ 心血管疾病的機理研究;⑷ 生物醫學電子學. 

         (四川大學 華西基礎醫學與法醫學院 生物醫學工程實驗室(成都 610041))
        摘要:研究剪切力對喉鱗癌細胞通透性變化的影響,初步探討力學刺激影響細胞生理功能的分子機制。方法對喉鱗癌Hep 2 細胞株施加1.4dyn/cm2 的剪切力刺激,倒置顯微鏡觀察不同時間點細胞的形態學變化;透射電鏡(TEM)觀察加載力學刺激和松弛后細胞胞間緊密連接的變化規律;Western blot 檢測緊密連接蛋白(Occludin、 Claudin-5 和ZO-1)的表達;共聚焦顯微鏡下觀察Claudin-5 分布及表達情況。結果 剪切力刺激后,細胞形態呈梭形,撤銷力學刺激后,細胞恢復至初始形態;TEM 觀察隨剪切力加載時間的增加細胞間連接更為密,通透性降低;剪切力誘導Occludin、Claudin-5 和ZO-1 的表達增加,撤銷剪切力后,其表達均呈現出顯著下調;免疫熒光結果表明Claudin-5 表達趨勢與Western blot 結果一致,靜態時Claudin-5 均勻分布在胞漿,加載剪切力易位于胞間,撤銷力學刺激后,在胞間和胞漿區域仍有Claudin-5 的表達。
        關鍵詞:流體剪切力;喉鱗癌;細胞通透性;緊密連接
        中圖分類號:中圖分類號Q27 文獻標識碼A
        The Effect of Fluid Shear Stress on the Permeability of Laryngeal Squamous Carcinoma Hep2 Cells
        Zhou Fating, Liu Xiaoheng, Shen Yang, LIU Shuangfeng, HONG Jinyong, XIA Qing
        (Institute of Biomedical Engineering,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041)
        Abstract: This paper is aimed to investigate the effect of fluid shear stress on the permeability of laryngeal squamous carcinoma (Hep2) cells and to explore the potential molecular mechanism. Hep2 cells were selected and subjected to the 1.4 dyn/cm2 fluid shear stress for different durations, respectively. The morphologic changes of Hep2 cells under shear stress were observed using inverted microscope. The cell-cell junctions were examined by transmission microscope (TEM). The
        expressions of tight junction proteins (including Occludin, Claudin-5 and ZO-1) and the distribution of Claudin-5 were examined by Western blot assay and laser scanning confocal microscope, respectively. The results showed that Hep2 cells showed spindle shapes after shear stress stimulation, and recovered to original polygon when shear stress was cancelled. The cell-cell junctions was tight under shear flow condition, the permeability was reduced under the condition of 1.4 dyn/cm2 shear flow. The expressions of tight junction proteins (Occludin, Claudin-5 and ZO-1) were enhanced with increased duration of shear flow, but reduced after removing shear flow. The result of Claudin-5 expression by immufluorescence assay was consistent with that by Western blot. The Claudin-5 mainly distributed in the cytoplasm under static condition, while it located at cell-cell junctions after shear flow stimulation. Therefore, we can conclude that shear stress mediates the change of the permeability of laryngeal squamous carcinoma Hep2 cells by by upregulation of the tight junction proteins such as Occludin,
         Claudin-5 and ZO-1.
        Key words: Fluid shear stress; Laryngeal squamous cell carcinoma; Permeability; Tight junction

        奇米色8888