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      1. 當前位置:首頁 > 智慧健康列表 > 認知神經科學與技術 > 詳細信息
        重組人粒細胞集落刺激因子在C57BL/6小鼠腸型急性放射病中的作用研究
        來源: | 作者: | 發布時間:2013-11-12 9:16:06

        重組人粒細胞集落刺激因子在C57BL/6小鼠腸型急性放射病中的作用研究
        劉偉1,齊丹丹1,顧永平2,崔鳳梅1,陳秋1
        基金項目:國家自然科學基金(81372920) 作者簡介:劉偉,(1988-),女,在讀研究生,研究方向:輻射防護 通信聯系人:陳秋,(1976-),女,副教授,研究方向:輻射防護.

        (1. 蘇州大學醫學部放射醫學與防護學院,蘇州,215123;2. 蘇州大學醫學部實驗中心,蘇州,215123)
        摘要:通過建立急性輻射損傷動物模型,研究重組人粒細胞集落刺激因子(rhG-CSF)在C57BL/6小鼠在腸型急性放射病中的作用。將健康C57BL/6小鼠隨機分成5組,分別是正常組、10Gy單純照射組及10Gy 照后rhG-CSF高、中、低濃度給藥組。照后觀察記錄小鼠的生存情況,繪制生存曲線;照后3.5d取骨組織、腸組織觀察形態學改變,并對腸組織進行Cleaved Caspase-3陽性細胞計數。實驗結果表明:10Gy全身照射后12h給予高濃度rhG-CSF,小鼠生存率提高顯著。照后3.5d,rhG-CSF高濃度給藥組與10Gy單純照射組比較,骨組織和腸組織絨毛長度變化不顯著,但隱窩凋亡細胞明顯減少。提示rhG-CSF可以減少小腸隱窩部位凋亡細胞,在C57BL/6小鼠腸型急性放射病中有一定保護作用。
        關鍵詞:重組人粒細胞集落刺激因子,腸型急性放射病,輻射防護
        中圖分類號:R818.05,R818.7
        Study on effect of rhG-CSF in intestinal form of acute radiation sickness in C57BL/6 mice
        Liu Wei1, Qi Dandan1, Gu Yongping2, Cui Fengmei1, Chen Qiu3
        (1. School of Radiation Medicine and Protection,Medical College of Soochow University,Suzhou,215123; 2. Experimental Centre,Medical College of Soochow University,Suzhou,215123; 3. School of Radiation Medicine and Protection ,Medical College of Soochow  University,Suzhou,215123)
        Abstract: To investigate effect of rhG-CSF in intestinal form of acute radiation sickness in C57BL/6 mice by the animal model of acute radiation damage, healthy C57BL/6 mice were randomly divided into 5 gropes, namely the normal grope, 10Gy radiation group, rhG-CSF administration group of high, middle, low concentration after 10Gy radiation . The kaplan-meier  survival curve of mice was drew after irradiation; 3.5days after radiation, bone and intestine were taken to observe the pathological change and the number of Cleaved Caspase-3 positive cells were counted. The results showed that 12 hours after 10Gy total body irradiation given the high concentration of rhG-CSF, the survival rate increased significantly. 3.5days after irradiation, comparing high concentrations of rhG-CSF administration group with 10Gy radiation alone group ,bone tissue and the length of intestinal villi did not change significantly, but the number of Cleaved Caspase-3 positive cells in crypts reduced . The results revealed that rhG-CSF reduced the number of Cleaved Caspase-3 positive cells in crypts having a protective role in the intestinal form of acute radiation sickness in C57BL / 6 mice.
        Key words: Recombinant human granulocyte colony stimulating factor; Intestinal form of acute  radiation sickness;Radioprotection

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