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      當前位置:首頁 > 智慧健康列表 > 生物材料研究 > 詳細信息
      腎素前體及其受體通過活性氧產生增多促進人臍動脈平滑肌細胞的增殖
      來源: | 作者: | 發布時間:2013-11-12 10:03:36

      腎素前體及其受體通過活性氧產生增多促進人臍動脈平滑肌細胞的增殖
      劉峰伊,劉曉云,張麗嬌,程云鵬,姜一農
      基金項目:高等學校博士學科點專項科研基金(20102105110006) 作者簡介:劉峰伊(1984-),女,博士研究生,主要研究方向:動脈粥樣硬化 通信聯系人:姜一農(1964-),女,教授,主要研究方向:動脈粥樣硬化的基礎研究.

      (大連醫科大學附屬第一醫院心內科,大連,116021)
      摘要:腎素(前體)受體(PRR)作為腎素-血管緊張素(RAS)系統的新成員,能夠不依賴于血管緊張素II(AngII)發揮作用,使人們對傳統的RAS系統有了新的認識。目的:研究腎素前體及其受體對人臍動脈平滑肌細胞(HUASMCs)增殖的影響及其可能的機制。方法:以原代培養的HUASMCs作為研究對象,用腎素前體干預HUASMCs,用流式細胞儀測定活性氧(ROS)的產生,用CCK-8測定細胞的增殖。結果:腎素前體與PRR結合可不依賴于AngII促進HUASMCs的增殖,同時腎素前體與PRR結合可不依賴于AngII可促進ROS產生增多。NADPH氧化酶抑制劑可顯著降低腎素前體引起的ROS升高,同時可明顯降低由腎素前體引起的HUASMCs的增殖。結論:腎素前體及其受體可不依賴于AngII促進ROS產生增多,并進一步促進HUASMCs的增殖。
      關鍵詞:腎素(前體)受體,活性氧,動脈粥樣硬化,血管平滑肌細胞增殖
      中圖分類號:R331.3+6
      Binging of prorenin to (pro)renin receptor induces the proliferation of human umbilical artery smooth muscle cells via ROS generation
      LIU Fengyi, LIU Xiaoyun, ZHANG Lijiao, CHENG Yunpeng, JIANG Yinong
      (Department of Cardiology, The First Affiliated Hospital of Dalian Medical University,Dalian,116021)
      Abstract: Since the discovery of (pro)renin receptor (PRR), it has been considered as a novel  bioactive molecule of the renin-angiotensin system (RAS). The activation of PRR can elicit a series of angiotensin II (AngII)-independent effects. Aim: To investigate the effects of prorenin and PRR on the proliferation of human umbilical artery smooth musclecells(HUASMCs) and explore the possible mechanisms underlying these effects. Methods: After stimulation with prorenin, we evaluated the proliferation of the HUASM cells by CCK-8 and the production of  reactive oxygen species (ROS) on a flow cytometer. Results: The binding of prorenin to PRR can promote proliferation independently of AngII in HUASM cells. In addition, the binding of prorenin to PRR can also increase the production of reactive oxygen species (ROS) independently of AngII. The pretreatment of HUASM cells with an NADPH oxidase inhibitor DPI decreased the production of ROS and also significantly attenuated the prorenin-induced proliferation.  Conclusion: Binding of prorenin to PRR can induce HUASM cells proliferation via the ROS generation.
      Key words: (pro)renin receptor, ROS, atherosclerosis, proliferation

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