摘要：種植體的表面微結構時決定細胞以及細菌行為的重要因素，但目前哪種微結構對上述行為有著積極影響尚不清楚。在本文中，我們分別在羥基磷灰石以及聚二甲基硅氧烷片上制備了不同形狀以及不同尺寸的微結構，并將C2C12 細胞以及S.a 、P.g 兩種細菌分別在上述兩種材料上培養，以分析細胞以及細菌在不同微結構上的表現。結果表明，C2C12 細胞在HA 上或者亞微米結構上生長較好，相反的，細菌更傾向于在微米級的結構表面生長。
Investigation of Adhesion and Proliferation Behavior of Myoblast and Bacteria on Micro-pattern Chip
Bao Chongyun, Xiao Yu
(State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University,Chengdu, 610041)Foundations: Natural Science Foundation of China (No. 81371181, No. 81171005)， Ministry of Education,Fund for the Doctoral (No. 20100181110058)
Brief author introduction:Bao Chongyun(1967-),Male, Professor, The main research field is in-vivo tissue engineering for bone regeneration.
Abstract: Surface microstructure of implant materials is an essential factor to determine the adhesion and proliferation behaviors of cells and bacteria. However, it is still unclear which kind of morphological characteristics plays dominant roles. For this purpose, hydroxyapatite (HA) and polydimethylsiloxane (PDMS) chips were prepared firstly with different types of microstructures and the C2C12 cells were seeded on these chips to investigate which microstructures could affect the
behavior of cells. Next, Staphylococcus aureus(S.a) and Porphyromonas gingivalis(P.g) were cultured on the PDMS matrix, respectively, to analyze the performance of bacteria on varied surface microstructures. The results illustrated much more C2C12 cells seeded on the HA chips than the PDMS 15 replicas, indicating HA are more suitable for the cell’s culture. Moreover, further observation and comparison represented both HA and PDMS samples illustrated a similar trend that the cells mainly adhered and proliferated on the submicron structure region rather than microstructure region. By contrast, the bacteria were significantly preferable to the microstructure regions. Overall, on account of different performance of cells and bacteria, we drew the conclusion that the surface roughness (RA) of implant materials should be manufactured in submicron-scale rather than micro-scale for improving the biocompatibility of biomaterials.
Key words: Basic Science of Stomatology; Microstructure; Hydroxyapatite; Cells Behavior;Roughness
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