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      來源: | 作者: | 發布時間:2014-4-15 15:59:05


       Preparation of Disk-like Natural Hydroxyapatite Samples and Cell Culture on Their Surfaces
      WANG Jiandan, ZHANG Zhiwei, Lü Xiaoying
      (State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096)
      Foundations: Doctoral Program of Higher Education of China (No.20100092110027); 973 Project (No. 2009CB930000); the National Natural Science Foundation of China (No. 31170910, 31271012, 30770583); the Graduate Research and Innovation Program of Jiangsu Province (No. CX10B-082Z) Brief author introduction:王健丹(1987-),女,碩士,主要研究方向:生物材料,生物相容性 Correspondance author: 呂曉迎(1956-),女,教授,主要研究方向:生物材料,生物相容性,生物電子學.

      Abstract: The purpose of this study is to prepare natural hydroxyapatite (NHA) for biocompatibility study. The NHA was prepared from pig bones by means of a calcining procedure, and then were made into disk-like samples. Subsequently mouse mesenchymal stem cells (MSCs) were cultured in vitro and the cell viability metabolism standard curve was obtained. Finally the mouse MSCs were also tried to seed on the surface of disk-like NHA samples. The result showed NHA could be derived from pig bones by a 650°C calcining procedure. MSCs viability metabolism standard curve was obtained and proper cell density of 6000 cells per wells was selected. The cells grew well on the surface of disk-like NHA samples. These results demonstrated that NHA have good biocompatibility.
      Key words: Biomaterial; Natural hydroxyapatite (NHA); Mesenchymal stem cells (MSCs); Cell  viability metabolism standard curve; Biocompatibility